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KMID : 0853020080110010032
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Journal of Korean Burn Society
2008 Volume.11 No. 1 p.32 ~ p.38
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The Effects of Botulinum Toxin A on the Survival of the Hair Bearing Composite Grafts
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Hong Jong-Won
Yoo Won-Min
Hahn Sung-Bum
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Abstract
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Purpose: In recent studies, Botulinum toxin A (BTA) has been received interest for sympathetic nerve block and has been reports on increased flap survival with use. In our study, we pretreated the recipient site with BTA and investigated the effect of increased composite graft survival on increased blood flow on recipient site.
Methods: On the 10 female SD rat, dorsal side was divided in to control site and experimental site. 2×2 cm sized defects were made on the caudal portion, same sized composite graft was harvested from the cephalic portion. On the experimental site, BTA 1 mL diluted to 10 U/mL was injected on the recipient site one week before the operation. From the
immediate preoperative time to postoperative 5 days, microcirculation on the operative field was measured with Doppler and composite tissue survival rate was photographed in digital image and survival rate was calculated. At postoperative 5
days, H/E staining was performed and vessel count, survival hair follicle was calculated.
Results: In the experimental site, the survival area was larger than in the control site, but there was no statistical significance. The preoperative microcirculation was more increased in the experimental site and postoperative microcirculation of composite graft showed no significant relevant between the experimental site and control site. In survived hair follicle, experimental site showed 3.2±0.4, control site showed 0.5±0.4 with statistical significance. In vessel count, experimental site showed 10.6±1.8 per HPF, contrast site showed 12.4±2.7. There was no statistical significance.
Conclusion: Pretreatment with BTA with increased the microcirculation of the recipient site showed increase in the number of survived hair follicle in the composite tissue graft, however, overall survival rate showed no difference between the experimental and control site. (Journal of Korean Burn Society 2008;11:32-38)
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KEYWORD
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BotulinumtoxinA, Hair, Compositegraft, Survival
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